{
"op": "seal",
"object": container
}
{
"op": "unseal",
"object": container
}
{
"op": "cover",
"object": container,
"lid": "standard" | "universal" |
"low_evaporation"
}
{
"op": "uncover",
"object": container
}Some instructions, such as the thermocycle or incubate instruction must be preceded by a seal or cover instruction.
On the other hand, if a container has a seal or a cover, it must be unsealed or uncovered before performing any action on the aliquots within. Autoprotocol-python makes this easy by automatically checking the cover status of a container before certain instructions and inserting a cover/seal (uncover/unseal) instruction where required.
Additionally, if executing a run as a package , a seal_on_store function is called at the end of the run which will apply a cover/seal instruction to all containers destined for storage, which are not already covered or sealed.
Some instructions can be run on covered or sealed containers, which are listed below:
pipette
yes
thermocycle
yes
absorbance
yes
yes
fluorescence
yes
yes
luminescence
yes
yes
incubate
yes
yes
gel_separate
yes
centrifugation
yes
yes
yes
For more information on covers and seals, see the Containers section.
In the case of PCR plates, the seal will be applied by an ALPS-3000 automatic sealer. Currently, optically clear heat seals are available. Chemically resistant foil seals will be added soon.
For plates that cannot be sealed a cover instruction can be used in which a lid type is specified. This instruction is completed by the gantry system.