Transcriptic

Cover and Seal

Instruction and Parameters

{
  "op": "seal",
  "object": container
}
{
  "op": "unseal",
  "object": container
}
{
  "op": "cover",
  "object": container,
  "lid": "standard" | "universal" |
         "low_evaporation"
}
{
  "op": "uncover",
  "object": container
}

Some instructions, such as the thermocycle or incubate instruction must be preceded by a seal or cover instruction.
On the other hand, if a container has a seal or a cover, it must be unsealed or uncovered before performing any action on the aliquots within. Autoprotocol-python makes this easy by automatically checking the cover status of a container before certain instructions and inserting a cover/seal (uncover/unseal) instruction where required.

Additionally, if executing a run as a package , a seal_on_store function is called at the end of the run which will apply a cover/seal instruction to all containers destined for storage, which are not already covered or sealed.

Some instructions can be run on covered or sealed containers, which are listed below:

Instruction
No cover?
Covered?
Sealed?

pipette

yes

thermocycle

yes

absorbance

yes

yes

fluorescence

yes

yes

luminescence

yes

yes

incubate

yes

yes

gel_separate

yes

centrifugation

yes

yes

yes

For more information on covers and seals, see the Containers section.

Device

In the case of PCR plates, the seal will be applied by an ALPS-3000 automatic sealer. Currently, optically clear heat seals are available. Chemically resistant foil seals will be added soon.

For plates that cannot be sealed a cover instruction can be used in which a lid type is specified. This instruction is completed by the gantry system.