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Cover and Seal

[block:api-header] { "type": "basic", "title": "Instruction and Parameters" } [/block] [block:code] { "codes": [ { "code": "{\n \"op\": \"seal\",\n \"object\": container\n}\n{\n \"op\": \"unseal\",\n \"object\": container\n}\n{\n \"op\": \"cover\",\n \"object\": container,\n \"lid\": \"standard\" | \"universal\" |\n \"low_evaporation\"\n}\n{\n \"op\": \"uncover\",\n \"object\": container\n}", "language": "json", "name": "JSON" } ], "sidebar": true } [/block] Some instructions, such as the `thermocycle` or `incubate` instruction must be preceded by a `seal` or `cover` instruction. On the other hand, if a container has a seal or a cover, it must be `unseal`ed or `uncover`ed before performing any action on the aliquots within. [Autoprotocol-python](https://github.com/autoprotocol/autoprotocol-python) makes this easy by automatically checking the cover status of a container before certain instructions and inserting a `cover/seal` (`uncover/unseal`) instruction where required. Additionally, if executing a run as a [package](doc:what-is-a-package) , a `seal_on_store` function is called at the end of the run which will apply a `cover/seal` instruction to all containers destined for storage, which are not already covered or sealed. Some instructions can be run on covered or sealed containers, which are listed below: [block:parameters] { "data": { "h-0": "Instruction", "h-1": "No cover?", "h-2": "Covered?", "h-3": "Sealed?", "0-0": "`pipette`", "1-0": "`thermocycle`", "2-0": "`absorbance`", "3-0": "fluorescence", "4-0": "`luminescence`", "5-0": "`incubate`", "6-0": "`gel_separate`", "7-0": "`centrifugation`", "6-1": "yes", "0-1": "yes", "1-3": "yes", "2-2": "yes", "2-1": "yes", "3-1": "yes", "3-2": "yes", "4-2": "yes", "4-1": "yes", "5-2": "yes", "5-3": "yes", "7-1": "yes", "7-2": "yes", "7-3": "yes" }, "cols": 4, "rows": 8 } [/block] For more information on covers and seals, see the [Containers](doc:containers) section. [block:api-header] { "type": "basic", "title": "Device" } [/block] In the case of PCR plates, the seal will be applied by an ALPS-3000 automatic sealer. Currently, optically clear heat seals are available. Chemically resistant foil seals will be added soon. For plates that cannot be `seal`ed a `cover` instruction can be used in which a lid type is specified. This instruction is completed by the gantry system.