{"__v":8,"_id":"571ab1eeee0bfe170037347c","category":{"__v":17,"_id":"568f0e73bdb9260d00149d8c","pages":["568f0ea29ebef90d0087276a","568f0eafb700ce0d002f4a32","568f0ebbbdb9260d00149d8d","568f0edeb700ce0d002f4a34","568f0ef3bdb9260d00149d8f","568f12099ebef90d0087276d","568f121abeb2700d00471839","568f20e49ebef90d0087277e","568f446d9ebef90d0087278f","56c79a728bf67e0d00734767","56cb8830c675f50b00a4b834","56d47e99a4a9211b00c8f0d2","56d4af6c1c4de4130005d74e","56d52a131c4de4130005d80b","56de001b3db43f2000d9a765","56de1087f25ce60e002e31ad","56e314c46e602e0e00700b35"],"project":"5476bf0f817e8d080031f988","version":"5476bf10817e8d080031f98b","sync":{"url":"","isSync":false},"reference":false,"createdAt":"2016-01-08T01:18:43.343Z","from_sync":false,"order":1,"slug":"protocols","title":"Premade Protocols"},"parentDoc":null,"project":"5476bf0f817e8d080031f988","user":"56f57b8eab3f610e000a6596","version":{"__v":17,"_id":"5476bf10817e8d080031f98b","project":"5476bf0f817e8d080031f988","createdAt":"2014-11-27T06:05:04.263Z","releaseDate":"2014-11-27T06:05:04.263Z","categories":["5476bf10817e8d080031f98c","5477c46cf3736008009e9eb5","5477c474f3736008009e9eb6","5477c47ef3736008009e9eb7","5477c48ff3736008009e9eb8","5477c4948deb230800808bf0","54e68328154f8e0d0007b55c","54e90194c8e0c00d007ac061","54eed2275bf74a0d00ef4076","54f7a7be0a3cbb0d00d666fb","559b0ebf7ae7f80d0096d871","55d697f9ae529e0d00d34f03","562d4dcc8c6e5a0d00d6ed1d","562e591c4376430d006f17e0","568f0e73bdb9260d00149d8c","5719542aac1e2e0e001834c6","57a14a8ed778850e0047e230"],"is_deprecated":false,"is_hidden":false,"is_beta":false,"is_stable":true,"codename":"","version_clean":"1.0.0","version":"1.0"},"updates":[],"next":{"pages":[],"description":""},"createdAt":"2016-04-22T23:21:18.957Z","link_external":false,"link_url":"","githubsync":"","sync_unique":"","hidden":true,"api":{"results":{"codes":[]},"settings":"","auth":"required","params":[],"url":""},"isReference":false,"order":2,"body":"[block:callout]\n{\n  \"type\": \"success\",\n  \"title\": \"This is a validated protocol\",\n  \"body\": \"The validation document for this protocol is available here\\n[http://learn.transcriptic.com/exosap/](http://learn.transcriptic.com/exosap/)\"\n}\n[/block]\n\n[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Description\"\n}\n[/block]\nThe ExoSAP-IT protocol uses a single-step enzymatic reaction to cleanup PCR products by eliminating unincorporated primers and dNTPs.\n[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Sample requirements\"\n}\n[/block]\nThis protocol can take samples in either of the following two formats:\n[block:parameters]\n{\n  \"data\": {\n    \"h-0\": \"Sample\",\n    \"h-1\": \"Explanation\",\n    \"0-0\": \"96 or 384-pcr plates to clean\",\n    \"1-0\": \"Samples to clean\",\n    \"0-1\": \"A 96-pcr or 384-pcr plate from your Inventory. The total volume of all samples will be purified.\",\n    \"1-1\": \"Individual aliquots from a 96-pcr or 384-pcr plate from your Inventory.\"\n  },\n  \"cols\": 2,\n  \"rows\": 2\n}\n[/block]\n\n[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Required parameters\"\n}\n[/block]\nParameters are only required if individual sample aliquots are selected.\n[block:parameters]\n{\n  \"data\": {\n    \"h-0\": \"Parameter\",\n    \"h-1\": \"Explanation\",\n    \"0-0\": \"Sample volume to purify\",\n    \"0-1\": \"Amount of the selected individual aliquots that will be purified.\"\n  },\n  \"cols\": 2,\n  \"rows\": 1\n}\n[/block]\n\n[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Protocol Outputs\"\n}\n[/block]\nThis protocol returns a 96-pcr plate or 384-pcr plate containing purified PCR product.\n[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Recommended guidelines\"\n}\n[/block]\nIt is recommended that this protocol only be used to purify PCR product, and not other samples of DNA with impurities. \n\nIf 96 or 384-pcr plates are selected as the sample inputs, the protocol will purify the entire volume of all aliquots in the plate. To instead purify a specific volume from all samples in the plate, the Pipetting Tools protocol can be used to transfer a specific amount into a new plate which can be used in this protocol. \n[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Further reading\"\n}\n[/block]\nMore information on the ExoSAP-IT kit can be found [here](http://www.affymetrix.com/catalog/131310/USB/ExoSAP-IT+For+PCR+Product+Cleanup#1_1).\n[block:callout]\n{\n  \"type\": \"success\",\n  \"body\": \"For further advice on your protocol please ask questions to the community at https://forum.transcriptic.com\",\n  \"title\": \"Check out the forum if you have more questions.\"\n}\n[/block]","excerpt":"","slug":"exosap-it","type":"basic","title":"ExoSAP-IT"}
[block:callout] { "type": "success", "title": "This is a validated protocol", "body": "The validation document for this protocol is available here\n[http://learn.transcriptic.com/exosap/](http://learn.transcriptic.com/exosap/)" } [/block] [block:api-header] { "type": "basic", "title": "Description" } [/block] The ExoSAP-IT protocol uses a single-step enzymatic reaction to cleanup PCR products by eliminating unincorporated primers and dNTPs. [block:api-header] { "type": "basic", "title": "Sample requirements" } [/block] This protocol can take samples in either of the following two formats: [block:parameters] { "data": { "h-0": "Sample", "h-1": "Explanation", "0-0": "96 or 384-pcr plates to clean", "1-0": "Samples to clean", "0-1": "A 96-pcr or 384-pcr plate from your Inventory. The total volume of all samples will be purified.", "1-1": "Individual aliquots from a 96-pcr or 384-pcr plate from your Inventory." }, "cols": 2, "rows": 2 } [/block] [block:api-header] { "type": "basic", "title": "Required parameters" } [/block] Parameters are only required if individual sample aliquots are selected. [block:parameters] { "data": { "h-0": "Parameter", "h-1": "Explanation", "0-0": "Sample volume to purify", "0-1": "Amount of the selected individual aliquots that will be purified." }, "cols": 2, "rows": 1 } [/block] [block:api-header] { "type": "basic", "title": "Protocol Outputs" } [/block] This protocol returns a 96-pcr plate or 384-pcr plate containing purified PCR product. [block:api-header] { "type": "basic", "title": "Recommended guidelines" } [/block] It is recommended that this protocol only be used to purify PCR product, and not other samples of DNA with impurities. If 96 or 384-pcr plates are selected as the sample inputs, the protocol will purify the entire volume of all aliquots in the plate. To instead purify a specific volume from all samples in the plate, the Pipetting Tools protocol can be used to transfer a specific amount into a new plate which can be used in this protocol. [block:api-header] { "type": "basic", "title": "Further reading" } [/block] More information on the ExoSAP-IT kit can be found [here](http://www.affymetrix.com/catalog/131310/USB/ExoSAP-IT+For+PCR+Product+Cleanup#1_1). [block:callout] { "type": "success", "body": "For further advice on your protocol please ask questions to the community at https://forum.transcriptic.com", "title": "Check out the forum if you have more questions." } [/block]