{"_id":"55d68d3bae529e0d00d34edb","parentDoc":null,"user":"54c096f492501c0d000b38a7","category":{"_id":"54f7a7be0a3cbb0d00d666fb","version":"5476bf10817e8d080031f98b","__v":19,"project":"5476bf0f817e8d080031f988","pages":["54f7a95354182d2100c9d057","54f7ad293607243500de2496","54f7ae7d0a3cbb0d00d66705","54f7b0013607243500de249b","54f7b4360a3cbb0d00d6670c","54f7c71154182d2100c9d073","54fa5205961fea21009206a9","54fa55e1c6db4517005b0147","54fddf642804410d00ee8a2a","5509fd2bdfed731900b81863","552dfa862594f70d001b2c48","552dfab3a702770d00d96d5b","552dfaca2594f70d001b2c4a","55d68d2c250d7d0d00427478","55d68d3bae529e0d00d34edb","5611dd433ca69417008981af","5679bca976cd370d003c1183","56de30df9ca83e17000cbc59","56eadf62199f7317006eb4db"],"sync":{"url":"","isSync":false},"reference":false,"createdAt":"2015-03-05T00:47:58.582Z","from_sync":false,"order":4,"slug":"instructions","title":"Capabilities"},"project":"5476bf0f817e8d080031f988","version":{"_id":"5476bf10817e8d080031f98b","__v":17,"project":"5476bf0f817e8d080031f988","createdAt":"2014-11-27T06:05:04.263Z","releaseDate":"2014-11-27T06:05:04.263Z","categories":["5476bf10817e8d080031f98c","5477c46cf3736008009e9eb5","5477c474f3736008009e9eb6","5477c47ef3736008009e9eb7","5477c48ff3736008009e9eb8","5477c4948deb230800808bf0","54e68328154f8e0d0007b55c","54e90194c8e0c00d007ac061","54eed2275bf74a0d00ef4076","54f7a7be0a3cbb0d00d666fb","559b0ebf7ae7f80d0096d871","55d697f9ae529e0d00d34f03","562d4dcc8c6e5a0d00d6ed1d","562e591c4376430d006f17e0","568f0e73bdb9260d00149d8c","5719542aac1e2e0e001834c6","57a14a8ed778850e0047e230"],"is_deprecated":false,"is_hidden":false,"is_beta":false,"is_stable":true,"codename":"","version_clean":"1.0.0","version":"1.0"},"__v":8,"updates":[],"next":{"pages":[],"description":""},"createdAt":"2015-08-21T02:30:19.914Z","link_external":false,"link_url":"","githubsync":"","sync_unique":"","hidden":false,"api":{"results":{"codes":[]},"settings":"","auth":"required","params":[],"url":""},"isReference":false,"order":1,"body":"Acoustic liquid handling of volumes below 2 microliters is performed using a [Labcyte Echo 525](http://www.labcyte.com/products/liquidhandling/echo-525-liquid-handler) Liquid Handler.   The default `droplet_size` of this device is fixed at 25nL and therefore all `acoustic_transfer` volumes should be integer multiples of 25nL.  Liquid may only be transferred from plates of type [`384-echo`](doc:containers).  Destination plates may be 96 or 384-well flat or PCR (v-bottom) plates. \n[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Instruction and Parameters\"\n}\n[/block]\n\n[block:code]\n{\n  \"codes\": [\n    {\n      \"code\": \"{\\n  \\\"op\\\": \\\"acoustic_transfer\\\",\\n  \\\"droplet_size\\\": volume\\n  \\\"groups\\\": [\\n    {\\n      \\\"transfer\\\": [\\n        {\\n          \\\"from\\\": well,\\n          \\\"to\\\": well,\\n        \\\"volume\\\": volume\\n        }\\n        ...\\n      ]\\n    }\\n  ],\\n}\",\n      \"language\": \"json\",\n      \"name\": \"acoustic_transfer\"\n    }\n  ]\n}\n[/block]","excerpt":"","slug":"liquid-handling-acoustic","type":"basic","title":"Liquid Handling - Acoustic"}

Liquid Handling - Acoustic


Acoustic liquid handling of volumes below 2 microliters is performed using a [Labcyte Echo 525](http://www.labcyte.com/products/liquidhandling/echo-525-liquid-handler) Liquid Handler. The default `droplet_size` of this device is fixed at 25nL and therefore all `acoustic_transfer` volumes should be integer multiples of 25nL. Liquid may only be transferred from plates of type [`384-echo`](doc:containers). Destination plates may be 96 or 384-well flat or PCR (v-bottom) plates. [block:api-header] { "type": "basic", "title": "Instruction and Parameters" } [/block] [block:code] { "codes": [ { "code": "{\n \"op\": \"acoustic_transfer\",\n \"droplet_size\": volume\n \"groups\": [\n {\n \"transfer\": [\n {\n \"from\": well,\n \"to\": well,\n \"volume\": volume\n }\n ...\n ]\n }\n ],\n}", "language": "json", "name": "acoustic_transfer" } ] } [/block]