{"_id":"5611dd433ca69417008981af","project":"5476bf0f817e8d080031f988","category":{"_id":"54f7a7be0a3cbb0d00d666fb","version":"5476bf10817e8d080031f98b","__v":19,"project":"5476bf0f817e8d080031f988","pages":["54f7a95354182d2100c9d057","54f7ad293607243500de2496","54f7ae7d0a3cbb0d00d66705","54f7b0013607243500de249b","54f7b4360a3cbb0d00d6670c","54f7c71154182d2100c9d073","54fa5205961fea21009206a9","54fa55e1c6db4517005b0147","54fddf642804410d00ee8a2a","5509fd2bdfed731900b81863","552dfa862594f70d001b2c48","552dfab3a702770d00d96d5b","552dfaca2594f70d001b2c4a","55d68d2c250d7d0d00427478","55d68d3bae529e0d00d34edb","5611dd433ca69417008981af","5679bca976cd370d003c1183","56de30df9ca83e17000cbc59","56eadf62199f7317006eb4db"],"sync":{"url":"","isSync":false},"reference":false,"createdAt":"2015-03-05T00:47:58.582Z","from_sync":false,"order":4,"slug":"instructions","title":"Capabilities"},"__v":10,"parentDoc":null,"user":"55e4edf4177b6e0d00333165","version":{"_id":"5476bf10817e8d080031f98b","__v":17,"project":"5476bf0f817e8d080031f988","createdAt":"2014-11-27T06:05:04.263Z","releaseDate":"2014-11-27T06:05:04.263Z","categories":["5476bf10817e8d080031f98c","5477c46cf3736008009e9eb5","5477c474f3736008009e9eb6","5477c47ef3736008009e9eb7","5477c48ff3736008009e9eb8","5477c4948deb230800808bf0","54e68328154f8e0d0007b55c","54e90194c8e0c00d007ac061","54eed2275bf74a0d00ef4076","54f7a7be0a3cbb0d00d666fb","559b0ebf7ae7f80d0096d871","55d697f9ae529e0d00d34f03","562d4dcc8c6e5a0d00d6ed1d","562e591c4376430d006f17e0","568f0e73bdb9260d00149d8c","5719542aac1e2e0e001834c6","57a14a8ed778850e0047e230"],"is_deprecated":false,"is_hidden":false,"is_beta":false,"is_stable":true,"codename":"","version_clean":"1.0.0","version":"1.0"},"updates":[],"next":{"pages":[],"description":""},"createdAt":"2015-10-05T02:15:31.285Z","link_external":false,"link_url":"","githubsync":"","sync_unique":"","hidden":false,"api":{"results":{"codes":[]},"settings":"","auth":"required","params":[],"url":""},"isReference":false,"order":3,"body":"Multichannel liquid handling at Transcriptic supports full 96-tip (8x12) as well as full rows (8xN) and full columns (Nx12) transfer in the SBS-compliant 96-tip format using the `stamp` instruction.\n[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Instruction and Parameters\"\n}\n[/block]\nThe `stamp` instruction has the field `groups`, which is an array of `transfer` objects\n[block:code]\n{\n  \"codes\": [\n    {\n      \"code\": \"{\\n  \\\"op\\\": \\\"stamp\\\",\\n  \\\"groups\\\": [\\n      {\\n        \\\"transfer\\\": [\\n          {\\n            \\\"from\\\": origin,\\n            \\\"to\\\": origin,\\n            \\\"volume\\\": volume,\\n\\n            // same mix options as a 'transfer' group in the 'pipette' instruction\\n            \\\"mix_before\\\": {...},  //optional\\n            \\\"mix_after\\\": {...} //optional\\n          },\\n          {...} // additional transfers using same tips\\n        ],\\n        \\\"shape\\\": {  // default: {\\\"rows\\\": 8, \\\"columns\\\": 12}\\n          \\\"rows\\\": integer,\\n          \\\"columns\\\": integer\\n        }, \\n        \\\"tip_layout\\\" : integer,  // optional, default: 96\\n      }\\n  ]\\n}\",\n      \"language\": \"json\",\n      \"name\": \"stamp\"\n    }\n  ]\n}\n[/block]\nEach `transfer` takes a `volume` of liquid `from` wells of a plate and transfers it `to` another plate using whole rows or whole columns of tips dependent on the `shape`.\n\nThe `from` and `to` wells correspond to the top-left wells of the source and destination respectively. \n\nThe `shape` parameter describes a rectangle with values `rows` and `columns` that specify the number of `rows` and `columns` of tips to be transferred in accordance to the SBS-compatible `tip_layout`. Transcriptic currently only supports `tip_layout = 96` and either `rows` must be 8 or `columns` must be 12 (only full rows or full columns can be transferred).\n\nEach `transfer` must have the same `shape` and `tip_layout`\n\nPlease be aware that `tip_layout = 96` will address a 384 well plate by skipping every other row and column.\n\nEvery `transfer` uses new tips. Instructions within a `transfer` use the same tips.\n[block:parameters]\n{\n  \"data\": {\n    \"h-0\": \"Volume Range\",\n    \"h-1\": \"%CV\",\n    \"0-0\": \"<5μL\",\n    \"1-0\": \"5μL - 200μL\",\n    \"2-0\": \"200μL - 1000μL\",\n    \"0-1\": \"2.1%\",\n    \"1-1\": \"1%\",\n    \"2-1\": \"1% or less\"\n  },\n  \"cols\": 2,\n  \"rows\": 3\n}\n[/block]\n## Tips\n\nThe tips used for stamp are automatically selected based on the total volume being moved in a single operation. Transfers within the same `stamp` group must use the same tip size.\n[block:parameters]\n{\n  \"data\": {\n    \"h-0\": \"Tip size\",\n    \"h-1\": \"Maximum volume\",\n    \"h-2\": \"Minimum volume\",\n    \"0-0\": \"Small\",\n    \"0-1\": \"31μL\",\n    \"0-2\": \"1μL\",\n    \"1-0\": \"Large\",\n    \"1-1\": \"110μL\",\n    \"1-2\": \"31μL\"\n  },\n  \"cols\": 3,\n  \"rows\": 2\n}\n[/block]\n\n[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Device\"\n}\n[/block]\nMultichannel liquid handling operations are performed using an Agilent Bravo liquid handler.","excerpt":"","slug":"liquid-handling-multi","type":"basic","title":"Liquid Handling - Multi Channel (Stamping)"}

Liquid Handling - Multi Channel (Stamping)


Multichannel liquid handling at Transcriptic supports full 96-tip (8x12) as well as full rows (8xN) and full columns (Nx12) transfer in the SBS-compliant 96-tip format using the `stamp` instruction. [block:api-header] { "type": "basic", "title": "Instruction and Parameters" } [/block] The `stamp` instruction has the field `groups`, which is an array of `transfer` objects [block:code] { "codes": [ { "code": "{\n \"op\": \"stamp\",\n \"groups\": [\n {\n \"transfer\": [\n {\n \"from\": origin,\n \"to\": origin,\n \"volume\": volume,\n\n // same mix options as a 'transfer' group in the 'pipette' instruction\n \"mix_before\": {...}, //optional\n \"mix_after\": {...} //optional\n },\n {...} // additional transfers using same tips\n ],\n \"shape\": { // default: {\"rows\": 8, \"columns\": 12}\n \"rows\": integer,\n \"columns\": integer\n }, \n \"tip_layout\" : integer, // optional, default: 96\n }\n ]\n}", "language": "json", "name": "stamp" } ] } [/block] Each `transfer` takes a `volume` of liquid `from` wells of a plate and transfers it `to` another plate using whole rows or whole columns of tips dependent on the `shape`. The `from` and `to` wells correspond to the top-left wells of the source and destination respectively. The `shape` parameter describes a rectangle with values `rows` and `columns` that specify the number of `rows` and `columns` of tips to be transferred in accordance to the SBS-compatible `tip_layout`. Transcriptic currently only supports `tip_layout = 96` and either `rows` must be 8 or `columns` must be 12 (only full rows or full columns can be transferred). Each `transfer` must have the same `shape` and `tip_layout` Please be aware that `tip_layout = 96` will address a 384 well plate by skipping every other row and column. Every `transfer` uses new tips. Instructions within a `transfer` use the same tips. [block:parameters] { "data": { "h-0": "Volume Range", "h-1": "%CV", "0-0": "<5μL", "1-0": "5μL - 200μL", "2-0": "200μL - 1000μL", "0-1": "2.1%", "1-1": "1%", "2-1": "1% or less" }, "cols": 2, "rows": 3 } [/block] ## Tips The tips used for stamp are automatically selected based on the total volume being moved in a single operation. Transfers within the same `stamp` group must use the same tip size. [block:parameters] { "data": { "h-0": "Tip size", "h-1": "Maximum volume", "h-2": "Minimum volume", "0-0": "Small", "0-1": "31μL", "0-2": "1μL", "1-0": "Large", "1-1": "110μL", "1-2": "31μL" }, "cols": 3, "rows": 2 } [/block] [block:api-header] { "type": "basic", "title": "Device" } [/block] Multichannel liquid handling operations are performed using an Agilent Bravo liquid handler.