{"__v":10,"_id":"571fe7f2a0acd42000af95ee","category":{"__v":19,"_id":"54f7a7be0a3cbb0d00d666fb","pages":["54f7a95354182d2100c9d057","54f7ad293607243500de2496","54f7ae7d0a3cbb0d00d66705","54f7b0013607243500de249b","54f7b4360a3cbb0d00d6670c","54f7c71154182d2100c9d073","54fa5205961fea21009206a9","54fa55e1c6db4517005b0147","54fddf642804410d00ee8a2a","5509fd2bdfed731900b81863","552dfa862594f70d001b2c48","552dfab3a702770d00d96d5b","552dfaca2594f70d001b2c4a","55d68d2c250d7d0d00427478","55d68d3bae529e0d00d34edb","5611dd433ca69417008981af","5679bca976cd370d003c1183","56de30df9ca83e17000cbc59","56eadf62199f7317006eb4db"],"project":"5476bf0f817e8d080031f988","version":"5476bf10817e8d080031f98b","sync":{"url":"","isSync":false},"reference":false,"createdAt":"2015-03-05T00:47:58.582Z","from_sync":false,"order":4,"slug":"instructions","title":"Capabilities"},"parentDoc":null,"project":"5476bf0f817e8d080031f988","user":"56f57b8eab3f610e000a6596","version":{"__v":17,"_id":"5476bf10817e8d080031f98b","project":"5476bf0f817e8d080031f988","createdAt":"2014-11-27T06:05:04.263Z","releaseDate":"2014-11-27T06:05:04.263Z","categories":["5476bf10817e8d080031f98c","5477c46cf3736008009e9eb5","5477c474f3736008009e9eb6","5477c47ef3736008009e9eb7","5477c48ff3736008009e9eb8","5477c4948deb230800808bf0","54e68328154f8e0d0007b55c","54e90194c8e0c00d007ac061","54eed2275bf74a0d00ef4076","54f7a7be0a3cbb0d00d666fb","559b0ebf7ae7f80d0096d871","55d697f9ae529e0d00d34f03","562d4dcc8c6e5a0d00d6ed1d","562e591c4376430d006f17e0","568f0e73bdb9260d00149d8c","5719542aac1e2e0e001834c6","57a14a8ed778850e0047e230"],"is_deprecated":false,"is_hidden":false,"is_beta":false,"is_stable":true,"codename":"","version_clean":"1.0.0","version":"1.0"},"updates":[],"next":{"pages":[],"description":""},"createdAt":"2016-04-26T22:13:06.254Z","link_external":false,"link_url":"","githubsync":"","sync_unique":"","hidden":false,"api":{"results":{"codes":[]},"settings":"","auth":"required","params":[],"url":""},"isReference":false,"order":12,"body":"[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Instructions and Parameters\"\n}\n[/block]\n##Measure Concentration\nReturns a dataref containing a `concentration` in the form of `X ng/uL` in addition to a `260/280 ratio` of type float. The concentration and quality will be appended as properties on the aliquot measured.\n[block:callout]\n{\n  \"type\": \"danger\",\n  \"title\": \"Beta\",\n  \"body\": \"This instruction is currently in beta development. Measurement of nucleic acid concentration using `measure_concentration` is under full control of autoprotocol, however, concentration measurement applications have not been validated on the system. Development of your own concentration measurements should proceed with care and caution. The Picogreen Assay can be used as an alternative method to measure DNA concentration.\"\n}\n[/block]\n\n[block:code]\n{\n  \"codes\": [\n    {\n      \"code\": \"{\\n    \\\"op\\\": \\\"measure_concentration\\\",\\n    \\\"object\\\": [aliquots],\\n    \\\"volume\\\": volume,   // default: \\\"2:microliter\\\"\\n    \\\"dataref\\\": string,\\n    \\\"measurement\\\": DNA | ssDNA | RNA | protein\\n}\",\n      \"language\": \"json\",\n      \"name\": \"measure_concentration\"\n    }\n  ]\n}\n[/block]\n##Measure Volume\nReturns a dataref containing a `volume` in the form of `X uL`. The volume will be updated on the aliquot measured. The `measure_volume` instruction should only be used on aliquots in `96-pcr`, `96-flat`, `micro-1.5`, or `micro-2.0` container types.\n[block:code]\n{\n  \"codes\": [\n    {\n      \"code\": \"{\\n    \\\"op\\\": \\\"measure_volume\\\",\\n    \\\"object\\\": [aliquots],\\n    \\\"dataref\\\": string\\n}\",\n      \"language\": \"json\",\n      \"name\": \"measure_volume\"\n    }\n  ]\n}\n[/block]\n##Measure Mass\nReturns a dataref containing a `mass` in the form of `X g`. The mass will be appended to the container measured.\n[block:code]\n{\n  \"codes\": [\n    {\n      \"code\": \"{\\n    \\\"op\\\": \\\"measure_mass\\\",\\n    \\\"object\\\": [containers],\\n    \\\"dataref\\\": string\\n}\",\n      \"language\": \"json\",\n      \"name\": \"measure_mass\"\n    }\n  ]\n}\n[/block]\n\n[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Device\"\n}\n[/block]\nThe `measure_concentration` instruction is handled on a NanoDrop Lite Spectrophotometer. The NanoDrop uses 1-2 μL of sample to measure the concentration of nucleic acids and purified protein. Nucleic acid concentration is measured at 260 nm and nucleic acid purity using the 260/280 nm ratio. Purified protein concentration is measured at 280 nm. \n\nMore information on the NanoDrop can be found [here](http://www.nanodrop.com/Productndliteoverview.aspx).\n\nThe `measure_volume` instruction is handled on the BioNex BeeSure Level Sensing System. More information on the BeeSure can be found [here](http://www.bionexsolutions.com/beesure.html).\n\nThe `measure_mass` instruction is handled on either a Intell-Lab PX-200 Analytical Balance or a Tree Electronic Precision Balance High Resolution Scale HRB-SG-602.\n\nThe manual for the Intell-Lab PX-200 can be found [here](https://www.intelligentwt.com/v/vspfiles/files/manuals/user-manuals/px-usermanual.pdf). More information on the Tree Electronic balance can be found [here](http://www.treescales.com/products/high-resolution-scale-with-shield/high-resolution-scale-with-shield-01).","excerpt":"","slug":"measurement-1","type":"basic","title":"Measurement"}
[block:api-header] { "type": "basic", "title": "Instructions and Parameters" } [/block] ##Measure Concentration Returns a dataref containing a `concentration` in the form of `X ng/uL` in addition to a `260/280 ratio` of type float. The concentration and quality will be appended as properties on the aliquot measured. [block:callout] { "type": "danger", "title": "Beta", "body": "This instruction is currently in beta development. Measurement of nucleic acid concentration using `measure_concentration` is under full control of autoprotocol, however, concentration measurement applications have not been validated on the system. Development of your own concentration measurements should proceed with care and caution. The Picogreen Assay can be used as an alternative method to measure DNA concentration." } [/block] [block:code] { "codes": [ { "code": "{\n \"op\": \"measure_concentration\",\n \"object\": [aliquots],\n \"volume\": volume, // default: \"2:microliter\"\n \"dataref\": string,\n \"measurement\": DNA | ssDNA | RNA | protein\n}", "language": "json", "name": "measure_concentration" } ] } [/block] ##Measure Volume Returns a dataref containing a `volume` in the form of `X uL`. The volume will be updated on the aliquot measured. The `measure_volume` instruction should only be used on aliquots in `96-pcr`, `96-flat`, `micro-1.5`, or `micro-2.0` container types. [block:code] { "codes": [ { "code": "{\n \"op\": \"measure_volume\",\n \"object\": [aliquots],\n \"dataref\": string\n}", "language": "json", "name": "measure_volume" } ] } [/block] ##Measure Mass Returns a dataref containing a `mass` in the form of `X g`. The mass will be appended to the container measured. [block:code] { "codes": [ { "code": "{\n \"op\": \"measure_mass\",\n \"object\": [containers],\n \"dataref\": string\n}", "language": "json", "name": "measure_mass" } ] } [/block] [block:api-header] { "type": "basic", "title": "Device" } [/block] The `measure_concentration` instruction is handled on a NanoDrop Lite Spectrophotometer. The NanoDrop uses 1-2 μL of sample to measure the concentration of nucleic acids and purified protein. Nucleic acid concentration is measured at 260 nm and nucleic acid purity using the 260/280 nm ratio. Purified protein concentration is measured at 280 nm. More information on the NanoDrop can be found [here](http://www.nanodrop.com/Productndliteoverview.aspx). The `measure_volume` instruction is handled on the BioNex BeeSure Level Sensing System. More information on the BeeSure can be found [here](http://www.bionexsolutions.com/beesure.html). The `measure_mass` instruction is handled on either a Intell-Lab PX-200 Analytical Balance or a Tree Electronic Precision Balance High Resolution Scale HRB-SG-602. The manual for the Intell-Lab PX-200 can be found [here](https://www.intelligentwt.com/v/vspfiles/files/manuals/user-manuals/px-usermanual.pdf). More information on the Tree Electronic balance can be found [here](http://www.treescales.com/products/high-resolution-scale-with-shield/high-resolution-scale-with-shield-01).