This is a validated protocol
View the validation data here
Description
This protocol orders DNA primers to be synthesized for use at Transcriptic. DNA is synthesized by IDT and will be available in your inventory once it is received and diluted per the experimental parameters.
gBlocks
gBlocks are larger fragments of double-strandedโ DNA that can be synthesized by IDT. Currently, the Oligosynthesis protocol does not support gBlocks, however they can be ordered via IDT and shipped directly to Transcriptic.
To add gBlocks to your inventory, follow the method outlined in this video. Make a note of the individual 3 digit tube codes, and the 4 digit Accessioning number for the shipping address.
Now go to IDT and order your gBlocks as you normally would, include the 3 digit labels at the end of the gBlock name in the order form. Next for the shipping address for the order use the address below but add your accessioning number
Transcriptic, Inc.
ATTN: Accessioning (Your accession number)
3565 Haven Avenue,
Suite 3,
Menlo Park
CA 94025
Sample requirements
This protocol does not require any sample inputs.
Required parameters
There are two ways to import sequences to be synthesised
Single oligonucleotides can be specified in the top portion of the protocol, however if you wish to synthesize a large number of oligonucleotides a bulk upload functionality is provided.


A screenshot of the oligosynthesis protocol launcher.
Single Oligonucleotides
sequence
specifies the sequence of DNA you wish to be synthesized, for example, ATGCGTAATCG
. N
is also an acceptable character for any base.
scale
specifies the quantity of DNA you wish to synthesize. Options are 10 nmol, 25 nmol, 100 nmol, 250 nmol and 1 ยตmol.
purification
specifies how you wish your synthesized oligonucleotides to be purified. Choose from Standard, HPLC and PAGE.
oligonucleotide name
specifies the name of your sequence.
diluent
specifies the solvent you wish your oligonucleotides to be diluted in. Choose from deionized water or TE buffer.
concentration
specifies the final concentration you wish your oligonucleotides to be diluted to.
More information on scale
and purification
can be found on the Oligosynthesis capabilities page.
Add additional sequences
If you wish to synthesize additional sequences click the
Add Oligonucleotide
link.
Bulk Upload Sequences
sequence CSV
the .csv
file you wish to upload that contains your sequence information. You can download a template of this here.
Universal parameters
This parameter is required for either synthesis method.
storage condition for diluted oligonucleotides
the conditions that you wish your diluted oligonucleotides to be stored in. Choose from -20 or 4 degree celsius.
Protocol Outputs
This protocol out puts physical samples that will be visible in your inventory once the run completes. The storage condition of the samples will be dependent on what you specified in your protocol.
Recommended guidelines
Sequences can be difficult to synthesize for various reasons, including GC content, homopolymers, length and secondary structure. It is recommend you review synthesis guidelines from IDT to advise your sequence choices, which are accessible here
Further reading
IDT
IDT Oligonucleotide synthesis FAQ
IDT Oligonucleotide purification FAQ
Check out the forum if you have more questions.
For further advice on your protocol please ask questions to the community at https://forum.transcriptic.com
Updated about a year ago