{"_id":"572b716981959f0e0087acee","parentDoc":null,"user":"56f57b8eab3f610e000a6596","__v":2,"category":{"_id":"568f0e73bdb9260d00149d8c","pages":["568f0ea29ebef90d0087276a","568f0eafb700ce0d002f4a32","568f0ebbbdb9260d00149d8d","568f0edeb700ce0d002f4a34","568f0ef3bdb9260d00149d8f","568f12099ebef90d0087276d","568f121abeb2700d00471839","568f20e49ebef90d0087277e","568f446d9ebef90d0087278f","56c79a728bf67e0d00734767","56cb8830c675f50b00a4b834","56d47e99a4a9211b00c8f0d2","56d4af6c1c4de4130005d74e","56d52a131c4de4130005d80b","56de001b3db43f2000d9a765","56de1087f25ce60e002e31ad","56e314c46e602e0e00700b35"],"__v":17,"project":"5476bf0f817e8d080031f988","version":"5476bf10817e8d080031f98b","sync":{"url":"","isSync":false},"reference":false,"createdAt":"2016-01-08T01:18:43.343Z","from_sync":false,"order":1,"slug":"protocols","title":"Premade Protocols"},"project":"5476bf0f817e8d080031f988","version":{"_id":"5476bf10817e8d080031f98b","__v":17,"project":"5476bf0f817e8d080031f988","createdAt":"2014-11-27T06:05:04.263Z","releaseDate":"2014-11-27T06:05:04.263Z","categories":["5476bf10817e8d080031f98c","5477c46cf3736008009e9eb5","5477c474f3736008009e9eb6","5477c47ef3736008009e9eb7","5477c48ff3736008009e9eb8","5477c4948deb230800808bf0","54e68328154f8e0d0007b55c","54e90194c8e0c00d007ac061","54eed2275bf74a0d00ef4076","54f7a7be0a3cbb0d00d666fb","559b0ebf7ae7f80d0096d871","55d697f9ae529e0d00d34f03","562d4dcc8c6e5a0d00d6ed1d","562e591c4376430d006f17e0","568f0e73bdb9260d00149d8c","5719542aac1e2e0e001834c6","57a14a8ed778850e0047e230"],"is_deprecated":false,"is_hidden":false,"is_beta":false,"is_stable":true,"codename":"","version_clean":"1.0.0","version":"1.0"},"updates":[],"next":{"pages":[],"description":""},"createdAt":"2016-05-05T16:14:33.484Z","link_external":false,"link_url":"","githubsync":"","sync_unique":"","hidden":false,"api":{"results":{"codes":[]},"settings":"","auth":"required","params":[],"url":""},"isReference":false,"order":13,"body":"[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Description\"\n}\n[/block]\nThis protocol will stamp liquid from a full rectangle of wells to a new or existing container. Only full rows or columns can be stamped, and the stamp will maintain the same shape of wells.\n[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Sample requirements\"\n}\n[/block]\n\n[block:parameters]\n{\n  \"data\": {\n    \"h-0\": \"Sample\",\n    \"h-1\": \"Explanation\",\n    \"0-0\": \"Source container\",\n    \"0-1\": \"A container of liquid sample that you wish to reformat into a new container.\",\n    \"1-0\": \"Destination plate (from inventory)\",\n    \"1-1\": \"A container that you wish to transfer sample into from your source container.\"\n  },\n  \"cols\": 2,\n  \"rows\": 2\n}\n[/block]\n\n[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Required parameters\"\n}\n[/block]\n\n[block:callout]\n{\n  \"type\": \"info\",\n  \"body\": \"Stamp uses a multichannel pipette to perform this liquid handling operation. To learn more about multichannel liquid handling and the `stamp` command please see [here](https://developers.transcriptic.com/docs/multichannel-liquid-handling-stamping).\",\n  \"title\": \"Stamp uses `stamp`\"\n}\n[/block]\n\n[block:parameters]\n{\n  \"data\": {\n    \"h-0\": \"Parameter\",\n    \"h-1\": \"Explanation\",\n    \"0-0\": \"Stamp Groups\",\n    \"2-0\": \"Transfer volume\",\n    \"3-0\": \"Destination\",\n    \"4-0\": \"New plate name (new container)\",\n    \"5-0\": \"Container type (new container)\",\n    \"1-0\": \"Source Wells - Full rows or columns\",\n    \"0-1\": \"A stamp group defines all parameters for a single stamp operation. Additional stamp operations may be specified by clicking the 'Add Stamp Groups' link at the bottom of the stamp group.\",\n    \"1-1\": \"Source wells must form a complete rectangle containing either entire rows or entire columns.\",\n    \"2-1\": \"The volume from the selected source wells to be stamped.\",\n    \"3-1\": \"Select either a new container or a container from your inventory to contain the transfer volume from the source quadrant.\\n\\nNew containers can be of type `96-pcr`, `96-flat`, `96-deep`, `384-pcr`, `384-flat`, or `384-echo`.\\n\\nFrom your inventory select a container.\",\n    \"4-1\": \"The name for the resulting new container.\",\n    \"5-1\": \"The container type for the new container. Select from `96-pcr`, `96-flat`, `96-deep`, `384-pcr`, `384-flat`, or `384-echo`.\",\n    \"6-0\": \"Storage condition\",\n    \"6-1\": \"The storage temperature you require for the destination container: either '-80 C', '-20 C', '4 C', or 'Room Temperature'.\",\n    \"7-0\": \"Top Left Destination Well\",\n    \"7-1\": \"The top left well of the rectangle in the destination plate to which the transfer volume from the source wells should be placed.\"\n  },\n  \"cols\": 2,\n  \"rows\": 8\n}\n[/block]\n\n[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Protocol Outputs\"\n}\n[/block]\nThis protocol outputs a either a new container or an existing container with the specified volume of liquid stamped from the source wells to the destination wells. New containers will be stored in your Inventory at the specified storage condition. Existing containers will be returned to the storage condition from which they came.\n\nNo measurement data is returned by this protocol.\n[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Recommended guidelines\"\n}\n[/block]\nEnsure you are aware of the dead volume in the source container. Dead volumes can be found in the containers section [here](https://developers.transcriptic.com/docs/containers#compatible-container-types). Failure to take into account dead volumes can result in insufficient volume being stamped.\n[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Further reading\"\n}\n[/block]\n\n[block:callout]\n{\n  \"type\": \"success\",\n  \"body\": \"For further advice on your protocol please ask questions to the community at https://forum.transcriptic.com\",\n  \"title\": \"Check out the forum if you have more questions.\"\n}\n[/block]","excerpt":"","slug":"pipetting-tools-row-column-stamp","type":"basic","title":"Pipetting Tools - Row & Column Stamp"}

Pipetting Tools - Row & Column Stamp


[block:api-header] { "type": "basic", "title": "Description" } [/block] This protocol will stamp liquid from a full rectangle of wells to a new or existing container. Only full rows or columns can be stamped, and the stamp will maintain the same shape of wells. [block:api-header] { "type": "basic", "title": "Sample requirements" } [/block] [block:parameters] { "data": { "h-0": "Sample", "h-1": "Explanation", "0-0": "Source container", "0-1": "A container of liquid sample that you wish to reformat into a new container.", "1-0": "Destination plate (from inventory)", "1-1": "A container that you wish to transfer sample into from your source container." }, "cols": 2, "rows": 2 } [/block] [block:api-header] { "type": "basic", "title": "Required parameters" } [/block] [block:callout] { "type": "info", "body": "Stamp uses a multichannel pipette to perform this liquid handling operation. To learn more about multichannel liquid handling and the `stamp` command please see [here](https://developers.transcriptic.com/docs/multichannel-liquid-handling-stamping).", "title": "Stamp uses `stamp`" } [/block] [block:parameters] { "data": { "h-0": "Parameter", "h-1": "Explanation", "0-0": "Stamp Groups", "2-0": "Transfer volume", "3-0": "Destination", "4-0": "New plate name (new container)", "5-0": "Container type (new container)", "1-0": "Source Wells - Full rows or columns", "0-1": "A stamp group defines all parameters for a single stamp operation. Additional stamp operations may be specified by clicking the 'Add Stamp Groups' link at the bottom of the stamp group.", "1-1": "Source wells must form a complete rectangle containing either entire rows or entire columns.", "2-1": "The volume from the selected source wells to be stamped.", "3-1": "Select either a new container or a container from your inventory to contain the transfer volume from the source quadrant.\n\nNew containers can be of type `96-pcr`, `96-flat`, `96-deep`, `384-pcr`, `384-flat`, or `384-echo`.\n\nFrom your inventory select a container.", "4-1": "The name for the resulting new container.", "5-1": "The container type for the new container. Select from `96-pcr`, `96-flat`, `96-deep`, `384-pcr`, `384-flat`, or `384-echo`.", "6-0": "Storage condition", "6-1": "The storage temperature you require for the destination container: either '-80 C', '-20 C', '4 C', or 'Room Temperature'.", "7-0": "Top Left Destination Well", "7-1": "The top left well of the rectangle in the destination plate to which the transfer volume from the source wells should be placed." }, "cols": 2, "rows": 8 } [/block] [block:api-header] { "type": "basic", "title": "Protocol Outputs" } [/block] This protocol outputs a either a new container or an existing container with the specified volume of liquid stamped from the source wells to the destination wells. New containers will be stored in your Inventory at the specified storage condition. Existing containers will be returned to the storage condition from which they came. No measurement data is returned by this protocol. [block:api-header] { "type": "basic", "title": "Recommended guidelines" } [/block] Ensure you are aware of the dead volume in the source container. Dead volumes can be found in the containers section [here](https://developers.transcriptic.com/docs/containers#compatible-container-types). Failure to take into account dead volumes can result in insufficient volume being stamped. [block:api-header] { "type": "basic", "title": "Further reading" } [/block] [block:callout] { "type": "success", "body": "For further advice on your protocol please ask questions to the community at https://forum.transcriptic.com", "title": "Check out the forum if you have more questions." } [/block]