{"__v":11,"_id":"568f121abeb2700d00471839","category":{"__v":17,"_id":"568f0e73bdb9260d00149d8c","pages":["568f0ea29ebef90d0087276a","568f0eafb700ce0d002f4a32","568f0ebbbdb9260d00149d8d","568f0edeb700ce0d002f4a34","568f0ef3bdb9260d00149d8f","568f12099ebef90d0087276d","568f121abeb2700d00471839","568f20e49ebef90d0087277e","568f446d9ebef90d0087278f","56c79a728bf67e0d00734767","56cb8830c675f50b00a4b834","56d47e99a4a9211b00c8f0d2","56d4af6c1c4de4130005d74e","56d52a131c4de4130005d80b","56de001b3db43f2000d9a765","56de1087f25ce60e002e31ad","56e314c46e602e0e00700b35"],"project":"5476bf0f817e8d080031f988","version":"5476bf10817e8d080031f98b","sync":{"url":"","isSync":false},"reference":false,"createdAt":"2016-01-08T01:18:43.343Z","from_sync":false,"order":1,"slug":"protocols","title":"Premade Protocols"},"parentDoc":null,"project":"5476bf0f817e8d080031f988","user":"568ed50cbeb2700d00471802","version":{"__v":17,"_id":"5476bf10817e8d080031f98b","project":"5476bf0f817e8d080031f988","createdAt":"2014-11-27T06:05:04.263Z","releaseDate":"2014-11-27T06:05:04.263Z","categories":["5476bf10817e8d080031f98c","5477c46cf3736008009e9eb5","5477c474f3736008009e9eb6","5477c47ef3736008009e9eb7","5477c48ff3736008009e9eb8","5477c4948deb230800808bf0","54e68328154f8e0d0007b55c","54e90194c8e0c00d007ac061","54eed2275bf74a0d00ef4076","54f7a7be0a3cbb0d00d666fb","559b0ebf7ae7f80d0096d871","55d697f9ae529e0d00d34f03","562d4dcc8c6e5a0d00d6ed1d","562e591c4376430d006f17e0","568f0e73bdb9260d00149d8c","5719542aac1e2e0e001834c6","57a14a8ed778850e0047e230"],"is_deprecated":false,"is_hidden":false,"is_beta":false,"is_stable":true,"codename":"","version_clean":"1.0.0","version":"1.0"},"updates":[],"next":{"pages":[],"description":""},"createdAt":"2016-01-08T01:34:18.636Z","link_external":false,"link_url":"","githubsync":"","sync_unique":"","hidden":true,"api":{"results":{"codes":[]},"settings":"","auth":"required","params":[],"url":""},"isReference":false,"order":17,"body":"[block:callout]\n{\n  \"type\": \"success\",\n  \"title\": \"This protocol is validated\",\n  \"body\": \"The validation document for this protocol is available here http://learn.transcriptic.com/plate-reading/\"\n}\n[/block]\n\n[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Description\"\n}\n[/block]\nThis protocol permits the ability to measure the absorbance, luminescence or fluorescence of samples in an optically-clear, flat-bottom plate. It will perform a measurement for each well in the provided plate.\n[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Sample requirements\"\n}\n[/block]\nThis protocol takes a single `container` as an input. This plate must be optically-clear and flat-bottomed. Compatible containers are `96-flat`, `96-flat-uv` and `384-flat`.\n[block:callout]\n{\n  \"type\": \"danger\",\n  \"body\": \"Non plate containers and some plate containers are not compatible with this protocol. For  a full list of container compatibility check https://developers.transcriptic.com/docs/containers\",\n  \"title\": \"Some containers are not compatible with this protocol\"\n}\n[/block]\n\n[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Required parameters\"\n}\n[/block]\nThis protocol provides a choice of different spectrophotometric analyses, hence the required parameters depending on which detection modality you employ in your run.\n[block:callout]\n{\n  \"type\": \"warning\",\n  \"body\": \"Take care to select the correct analysis technique for your samples. Particularly with fluorescence ensure you have your excitation and emission wavelengths the correct way around.\",\n  \"title\": \"Understand your parameters\"\n}\n[/block]\n`measurement_type` Choose from a choice of either `absorbance`, `luminescence` and `fluorescence` depending on your application.\n\n## Absorbance\n\n`wavelength` The wavelength of light that you wish to use in your analysis. The acceptable range is 300 nm to 1000 nm.\n\n## Luminescence\n\n`wavelength` The wavelength of light that you wish to use in your analysis. The acceptable range is 380 nm - 600 nm.\n\n## Fluorescence\n\n`fluorescence excitation wavelength` The excitation wavelength compatible with your fluorophore, the acceptable range is 230 nm - 600 nm.\n\n`fluorescence emission wavelength` The emission wavelength of your fluorophore you wish to measure. The acceptable range is 330 nm - 850 nm.\n\n[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Protocol Outputs\"\n}\n[/block]\nThis protocol returns a `.csv` file containing the results as list of each measurement obtained for each well. A visualization of measurement 'intensity' is also provided in the run view under the 'Results' tab.\n[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Recommended Guidelines\"\n}\n[/block]\nFluorescent mode gain is not exposed as a parameter to the user. The instrument instead is set to *optimal gain* mode: Calculated automatically by the instrument according to the highest signal within the selected well range in order to avoid OVER. Optimal gain determination is performed in a pre-measurement. It is recommended to use the optimal gain function for all applications that produce results with unknown RFU values.\n[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Further reading\"\n}\n[/block]\nIf you wish to incorporate plate reading into your custom protocols see the developer documentation [here](https://developers.transcriptic.com/docs/spectophotometry)\nFor information about container compatibility check [here](https://developers.transcriptic.com/docs/containers) \n[block:callout]\n{\n  \"type\": \"success\",\n  \"body\": \"For further advice on your protocol please ask questions to the community at https://forum.transcriptic.com\",\n  \"title\": \"Check out the forum if you have more questions.\"\n}\n[/block]","excerpt":"","slug":"plate-reading","type":"basic","title":"Plate Reading"}
[block:callout] { "type": "success", "title": "This protocol is validated", "body": "The validation document for this protocol is available here http://learn.transcriptic.com/plate-reading/" } [/block] [block:api-header] { "type": "basic", "title": "Description" } [/block] This protocol permits the ability to measure the absorbance, luminescence or fluorescence of samples in an optically-clear, flat-bottom plate. It will perform a measurement for each well in the provided plate. [block:api-header] { "type": "basic", "title": "Sample requirements" } [/block] This protocol takes a single `container` as an input. This plate must be optically-clear and flat-bottomed. Compatible containers are `96-flat`, `96-flat-uv` and `384-flat`. [block:callout] { "type": "danger", "body": "Non plate containers and some plate containers are not compatible with this protocol. For a full list of container compatibility check https://developers.transcriptic.com/docs/containers", "title": "Some containers are not compatible with this protocol" } [/block] [block:api-header] { "type": "basic", "title": "Required parameters" } [/block] This protocol provides a choice of different spectrophotometric analyses, hence the required parameters depending on which detection modality you employ in your run. [block:callout] { "type": "warning", "body": "Take care to select the correct analysis technique for your samples. Particularly with fluorescence ensure you have your excitation and emission wavelengths the correct way around.", "title": "Understand your parameters" } [/block] `measurement_type` Choose from a choice of either `absorbance`, `luminescence` and `fluorescence` depending on your application. ## Absorbance `wavelength` The wavelength of light that you wish to use in your analysis. The acceptable range is 300 nm to 1000 nm. ## Luminescence `wavelength` The wavelength of light that you wish to use in your analysis. The acceptable range is 380 nm - 600 nm. ## Fluorescence `fluorescence excitation wavelength` The excitation wavelength compatible with your fluorophore, the acceptable range is 230 nm - 600 nm. `fluorescence emission wavelength` The emission wavelength of your fluorophore you wish to measure. The acceptable range is 330 nm - 850 nm. [block:api-header] { "type": "basic", "title": "Protocol Outputs" } [/block] This protocol returns a `.csv` file containing the results as list of each measurement obtained for each well. A visualization of measurement 'intensity' is also provided in the run view under the 'Results' tab. [block:api-header] { "type": "basic", "title": "Recommended Guidelines" } [/block] Fluorescent mode gain is not exposed as a parameter to the user. The instrument instead is set to *optimal gain* mode: Calculated automatically by the instrument according to the highest signal within the selected well range in order to avoid OVER. Optimal gain determination is performed in a pre-measurement. It is recommended to use the optimal gain function for all applications that produce results with unknown RFU values. [block:api-header] { "type": "basic", "title": "Further reading" } [/block] If you wish to incorporate plate reading into your custom protocols see the developer documentation [here](https://developers.transcriptic.com/docs/spectophotometry) For information about container compatibility check [here](https://developers.transcriptic.com/docs/containers) [block:callout] { "type": "success", "body": "For further advice on your protocol please ask questions to the community at https://forum.transcriptic.com", "title": "Check out the forum if you have more questions." } [/block]