{"__v":1,"_id":"57e355444d659c0e0006e8ed","category":{"__v":19,"_id":"54f7a7be0a3cbb0d00d666fb","pages":["54f7a95354182d2100c9d057","54f7ad293607243500de2496","54f7ae7d0a3cbb0d00d66705","54f7b0013607243500de249b","54f7b4360a3cbb0d00d6670c","54f7c71154182d2100c9d073","54fa5205961fea21009206a9","54fa55e1c6db4517005b0147","54fddf642804410d00ee8a2a","5509fd2bdfed731900b81863","552dfa862594f70d001b2c48","552dfab3a702770d00d96d5b","552dfaca2594f70d001b2c4a","55d68d2c250d7d0d00427478","55d68d3bae529e0d00d34edb","5611dd433ca69417008981af","5679bca976cd370d003c1183","56de30df9ca83e17000cbc59","56eadf62199f7317006eb4db"],"project":"5476bf0f817e8d080031f988","version":"5476bf10817e8d080031f98b","sync":{"url":"","isSync":false},"reference":false,"createdAt":"2015-03-05T00:47:58.582Z","from_sync":false,"order":4,"slug":"instructions","title":"Capabilities"},"parentDoc":null,"project":"5476bf0f817e8d080031f988","user":"55e4edf4177b6e0d00333165","version":{"__v":17,"_id":"5476bf10817e8d080031f98b","project":"5476bf0f817e8d080031f988","createdAt":"2014-11-27T06:05:04.263Z","releaseDate":"2014-11-27T06:05:04.263Z","categories":["5476bf10817e8d080031f98c","5477c46cf3736008009e9eb5","5477c474f3736008009e9eb6","5477c47ef3736008009e9eb7","5477c48ff3736008009e9eb8","5477c4948deb230800808bf0","54e68328154f8e0d0007b55c","54e90194c8e0c00d007ac061","54eed2275bf74a0d00ef4076","54f7a7be0a3cbb0d00d666fb","559b0ebf7ae7f80d0096d871","55d697f9ae529e0d00d34f03","562d4dcc8c6e5a0d00d6ed1d","562e591c4376430d006f17e0","568f0e73bdb9260d00149d8c","5719542aac1e2e0e001834c6","57a14a8ed778850e0047e230"],"is_deprecated":false,"is_hidden":false,"is_beta":false,"is_stable":true,"codename":"","version_clean":"1.0.0","version":"1.0"},"updates":[],"next":{"pages":[],"description":""},"createdAt":"2016-09-22T03:51:32.158Z","link_external":false,"link_url":"","githubsync":"","sync_unique":"","hidden":false,"api":{"settings":"","results":{"codes":[]},"auth":"required","params":[],"url":""},"isReference":false,"order":17,"body":"[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Instruction and Parameters\"\n}\n[/block]\n\n[block:code]\n{\n  \"codes\": [\n    {\n      \"code\": \"{\\n  \\\"op\\\": \\\"dispense\\\",\\n  \\\"object\\\": \\\"sample_plate\\\",\\n  \\\"resource_id\\\": \\\"rs17gmh5wafm5p\\\",\\n  \\\"columns\\\": [\\n    { \\\"column\\\": 0, \\\"volume\\\": \\\"50:microliter\\\" },\\n    { \\\"column\\\": 4, \\\"volume\\\": \\\"100:microliter\\\" },\\n    { \\\"column\\\": 8, \\\"volume\\\": \\\"1:milliliter\\\" },\\n    { \\\"column\\\": 11, \\\"volume\\\": \\\"2:milliliter\\\" }\\n  ]\\n}\",\n      \"language\": \"json\",\n      \"name\": \"dispense\"\n    }\n  ],\n  \"sidebar\": true\n}\n[/block]\nTranscriptic carries a set of standard reagents that can be distributed onto a plate. Reagents are dispensed in 5uL increments from 250mL or 500mL bottles. These bottles have a dead volume of approximately 5 mL, which means the last 5 mL cannot be used.\n\nBefore a given reagent dispenser can be used during a run, it must be primed to ensure accurate volume dispensing. This process consumes an additional 2 mL of reagent. Priming must be repeated after 6 hours of inactivity.\n\nAvailable reagents are listed below, along with their corresponding resource ids:\n[block:html]\n{\n  \"html\": \"<style>dd {\\n    margin-bottom: 10px;\\n  }\\n  dl {\\n    padding-left: 10px;\\n    border-left: 3px solid #eee;\\n    dt code {\\n      font-weight: normal;\\n    }\\n  }\\n</style>\\n<dl>\\n\\t<dt>DI H2O</dt> <dd><code>rs17gmh5wafm5p</code><dd>\\n\\t<dt>1x PBS</dt> <dd><code>rs194na2u3hfam</code><dd>\\n  <dt>100 ug/ml Orange G dye</dt> <dd><code>rs17zw9zsaqd55</code><dd>(other dye concentrations will not be supported moving forward, except for legacy protocols)<dd> \\n</dl>\"\n}\n[/block]\n\n[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Device\"\n}\n[/block]\nThis instruction uses the Multidrop Combi Reagent Dispenser from Thermo Scientific. \nIn its configuration at Transcriptic it supports a volume range from 5uL to 2ml and can support all plate types. A 96 well plate can be filled with 20uL of solution in 4 seconds, a 384 well plate in 9 seconds. \n\nWe observe the following dispensing precisions and accuracies:\n[block:parameters]\n{\n  \"data\": {\n    \"0-0\": \"5uL\",\n    \"2-0\": \"100uL\",\n    \"3-0\": \"\\\\> 100uL\",\n    \"0-1\": \"≤ 10%\",\n    \"2-1\": \"≤ 1%\",\n    \"3-1\": \"≤ 1%\",\n    \"0-2\": \"± 10%\",\n    \"2-2\": \"± 1%\",\n    \"3-2\": \"± 1%\",\n    \"h-0\": \"Volume\",\n    \"h-1\": \"Precision (Coefficient of Variation)\",\n    \"h-2\": \"Accuracy\",\n    \"1-0\": \"20uL\",\n    \"1-1\": \"≤ 1.5%\",\n    \"1-2\": \"± 2%\"\n  },\n  \"cols\": 3,\n  \"rows\": 4\n}\n[/block]","excerpt":"","slug":"reagent-dispensing","type":"basic","title":"Reagent Dispensing"}

Reagent Dispensing


[block:api-header] { "type": "basic", "title": "Instruction and Parameters" } [/block] [block:code] { "codes": [ { "code": "{\n \"op\": \"dispense\",\n \"object\": \"sample_plate\",\n \"resource_id\": \"rs17gmh5wafm5p\",\n \"columns\": [\n { \"column\": 0, \"volume\": \"50:microliter\" },\n { \"column\": 4, \"volume\": \"100:microliter\" },\n { \"column\": 8, \"volume\": \"1:milliliter\" },\n { \"column\": 11, \"volume\": \"2:milliliter\" }\n ]\n}", "language": "json", "name": "dispense" } ], "sidebar": true } [/block] Transcriptic carries a set of standard reagents that can be distributed onto a plate. Reagents are dispensed in 5uL increments from 250mL or 500mL bottles. These bottles have a dead volume of approximately 5 mL, which means the last 5 mL cannot be used. Before a given reagent dispenser can be used during a run, it must be primed to ensure accurate volume dispensing. This process consumes an additional 2 mL of reagent. Priming must be repeated after 6 hours of inactivity. Available reagents are listed below, along with their corresponding resource ids: [block:html] { "html": "<style>dd {\n margin-bottom: 10px;\n }\n dl {\n padding-left: 10px;\n border-left: 3px solid #eee;\n dt code {\n font-weight: normal;\n }\n }\n</style>\n<dl>\n\t<dt>DI H2O</dt> <dd><code>rs17gmh5wafm5p</code><dd>\n\t<dt>1x PBS</dt> <dd><code>rs194na2u3hfam</code><dd>\n <dt>100 ug/ml Orange G dye</dt> <dd><code>rs17zw9zsaqd55</code><dd>(other dye concentrations will not be supported moving forward, except for legacy protocols)<dd> \n</dl>" } [/block] [block:api-header] { "type": "basic", "title": "Device" } [/block] This instruction uses the Multidrop Combi Reagent Dispenser from Thermo Scientific. In its configuration at Transcriptic it supports a volume range from 5uL to 2ml and can support all plate types. A 96 well plate can be filled with 20uL of solution in 4 seconds, a 384 well plate in 9 seconds. We observe the following dispensing precisions and accuracies: [block:parameters] { "data": { "0-0": "5uL", "2-0": "100uL", "3-0": "\\> 100uL", "0-1": "≤ 10%", "2-1": "≤ 1%", "3-1": "≤ 1%", "0-2": "± 10%", "2-2": "± 1%", "3-2": "± 1%", "h-0": "Volume", "h-1": "Precision (Coefficient of Variation)", "h-2": "Accuracy", "1-0": "20uL", "1-1": "≤ 1.5%", "1-2": "± 2%" }, "cols": 3, "rows": 4 } [/block]