Transcriptic

Reagent Dispensing

Instruction and Parameters

{
  "op": "dispense",
  "object": "sample_plate",
  "resource_id": "rs17gmh5wafm5p",
  "columns": [
    { "column": 0, "volume": "50:microliter" },
    { "column": 4, "volume": "100:microliter" },
    { "column": 8, "volume": "1:milliliter" },
    { "column": 11, "volume": "2:milliliter" }
  ]
}

Transcriptic carries a set of standard reagents that can be distributed onto a plate. Reagents are dispensed in 5uL increments from 250mL or 500mL bottles. These bottles have a dead volume of approximately 5 mL, which means the last 5 mL cannot be used.

Before a given reagent dispenser can be used during a run, it must be primed to ensure accurate volume dispensing. This process consumes an additional 2 mL of reagent. Priming must be repeated after 6 hours of inactivity.

Available reagents are listed below, along with their corresponding resource ids:

DI H2O
rs17gmh5wafm5p
1x PBS
rs194na2u3hfam
100 ug/ml Orange G dye
rs17zw9zsaqd55
(other dye concentrations will not be supported moving forward, except for legacy protocols)

Device

This instruction uses the Multidrop Combi Reagent Dispenser from Thermo Scientific.
In its configuration at Transcriptic it supports a volume range from 5uL to 2ml in 5uL increments, and can support all plate types. A 96 well plate can be filled with 20uL of solution in 4 seconds, a 384 well plate in 9 seconds.

We observe the following dispensing precisions and accuracies:

Volume
Precision (Coefficient of Variation)
Accuracy

5uL

≤ 10%

± 10%

20uL

≤ 1.5%

± 2%

100uL

≤ 1%

± 1%

> 100uL

≤ 1%

± 1%

Reagent Dispensing


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