{"_id":"56d47e99a4a9211b00c8f0d2","parentDoc":null,"user":"568ed50cbeb2700d00471802","version":{"_id":"5476bf10817e8d080031f98b","__v":17,"project":"5476bf0f817e8d080031f988","createdAt":"2014-11-27T06:05:04.263Z","releaseDate":"2014-11-27T06:05:04.263Z","categories":["5476bf10817e8d080031f98c","5477c46cf3736008009e9eb5","5477c474f3736008009e9eb6","5477c47ef3736008009e9eb7","5477c48ff3736008009e9eb8","5477c4948deb230800808bf0","54e68328154f8e0d0007b55c","54e90194c8e0c00d007ac061","54eed2275bf74a0d00ef4076","54f7a7be0a3cbb0d00d666fb","559b0ebf7ae7f80d0096d871","55d697f9ae529e0d00d34f03","562d4dcc8c6e5a0d00d6ed1d","562e591c4376430d006f17e0","568f0e73bdb9260d00149d8c","5719542aac1e2e0e001834c6","57a14a8ed778850e0047e230"],"is_deprecated":false,"is_hidden":false,"is_beta":false,"is_stable":true,"codename":"","version_clean":"1.0.0","version":"1.0"},"__v":16,"category":{"_id":"568f0e73bdb9260d00149d8c","pages":["568f0ea29ebef90d0087276a","568f0eafb700ce0d002f4a32","568f0ebbbdb9260d00149d8d","568f0edeb700ce0d002f4a34","568f0ef3bdb9260d00149d8f","568f12099ebef90d0087276d","568f121abeb2700d00471839","568f20e49ebef90d0087277e","568f446d9ebef90d0087278f","56c79a728bf67e0d00734767","56cb8830c675f50b00a4b834","56d47e99a4a9211b00c8f0d2","56d4af6c1c4de4130005d74e","56d52a131c4de4130005d80b","56de001b3db43f2000d9a765","56de1087f25ce60e002e31ad","56e314c46e602e0e00700b35"],"__v":17,"project":"5476bf0f817e8d080031f988","version":"5476bf10817e8d080031f98b","sync":{"url":"","isSync":false},"reference":false,"createdAt":"2016-01-08T01:18:43.343Z","from_sync":false,"order":1,"slug":"protocols","title":"Premade Protocols"},"project":"5476bf0f817e8d080031f988","updates":[],"next":{"pages":[],"description":""},"createdAt":"2016-02-29T17:23:37.842Z","link_external":false,"link_url":"","githubsync":"","sync_unique":"","hidden":false,"api":{"results":{"codes":[]},"settings":"","auth":"required","params":[],"url":""},"isReference":false,"order":11,"body":"[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Description\"\n}\n[/block]\nThis protocol takes a volume of an aliquot and dilutes it into a new container with a choice of diluent from the Transcriptic reagent library or from your own inventory.\n[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Sample requirements\"\n}\n[/block]\n\n[block:parameters]\n{\n  \"data\": {\n    \"h-0\": \"Sample\",\n    \"h-1\": \"Explanation\",\n    \"0-0\": \"Sample to dilute\",\n    \"0-1\": \"An aliquot of a liquid sample that you wish to have diluted.\",\n    \"1-0\": \"Diluent (from inventory)\",\n    \"1-1\": \"An aliquot of a liquid sample that you wish to use to dilute your other sample.\"\n  },\n  \"cols\": 2,\n  \"rows\": 2\n}\n[/block]\n\n[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Required parameters\"\n}\n[/block]\n\n[block:callout]\n{\n  \"type\": \"info\",\n  \"body\": \"Dilute uses a single channel pipette to perform the liquid handling. To learn more about single channel liquid handling and the `transfer` command please see [here](https://developers.transcriptic.com/docs/liquid-handling#section-transfer)\",\n  \"title\": \"Dilute uses `transfer`\"\n}\n[/block]\n\n[block:parameters]\n{\n  \"data\": {\n    \"h-0\": \"Parameter\",\n    \"h-1\": \"Explanation\",\n    \"0-0\": \"Sample (group)\",\n    \"2-0\": \"Volume of sample\",\n    \"3-0\": \"Volume of diluent\",\n    \"4-0\": \"Tube type for diluted aliquot\",\n    \"5-0\": \"Storage condition for diluted aliquot\",\n    \"1-0\": \"Container label\",\n    \"0-1\": \"A sample group defines all parameters for a single dilution. Additional dilutions may be specified by clicking the 'Add Sample' link at the bottom of the Sample group.\",\n    \"1-1\": \"The name for the new resulting diluted aliquot. Aliquot names associated with this container will be derived from the source aliquot name.\",\n    \"2-1\": \"The volume of sample to be diluted.\",\n    \"3-1\": \"The volume of diluent to be added to the volume of sample to be diluted.\",\n    \"4-1\": \"The new container for the newly diluted aliquot. Compatible containers: `micro-1.5`, `micro-2.0`, `96-pcr`, `96-deep`, `96-flat`, `384-pcr`,  `384-flat`,  `384-echo`.\",\n    \"5-1\": \"The storage temperature you require for the diluted sample: either `cold_20`, `cold_4` or `ambient`.\",\n    \"6-0\": \"Diluent\",\n    \"6-1\": \"Select either a diluent from the reagent library or from your inventory.\\n\\nFrom the library both water and TE Buffer are available.\\n\\nFrom your inventory select an aliquot.\"\n  },\n  \"cols\": 2,\n  \"rows\": 7\n}\n[/block]\n\n[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Protocol Outputs\"\n}\n[/block]\nThis protocol outputs a new aliquot of the diluted sample which is stored in your inventory at your specified storage condition. Any aliquots used in the making of the dilutions will be returned to the storage condition from which they came.\n\nNo measurement data is returned by this protocol.\n[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Recommended guidelines\"\n}\n[/block]\nEnsure you are aware of the dead volume in the containers you are using for both the sample to be diluted and the diluent (if you are using a diluent from your inventory). Dead volumes can be found in the containers section [here](https://developers.transcriptic.com/docs/containers#compatible-container-types). Failure to take into account dead volumes can result in insufficient volume being pipetted.\n[block:api-header]\n{\n  \"type\": \"basic\",\n  \"title\": \"Further reading\"\n}\n[/block]\n\n[block:callout]\n{\n  \"type\": \"success\",\n  \"body\": \"For further advice on your protocol please ask questions to the community at https://forum.transcriptic.com\",\n  \"title\": \"Check out the forum if you have more questions.\"\n}\n[/block]","excerpt":"","slug":"singledilute-1","type":"basic","title":"Pipetting Tools - Dilute"}

Pipetting Tools - Dilute


[block:api-header] { "type": "basic", "title": "Description" } [/block] This protocol takes a volume of an aliquot and dilutes it into a new container with a choice of diluent from the Transcriptic reagent library or from your own inventory. [block:api-header] { "type": "basic", "title": "Sample requirements" } [/block] [block:parameters] { "data": { "h-0": "Sample", "h-1": "Explanation", "0-0": "Sample to dilute", "0-1": "An aliquot of a liquid sample that you wish to have diluted.", "1-0": "Diluent (from inventory)", "1-1": "An aliquot of a liquid sample that you wish to use to dilute your other sample." }, "cols": 2, "rows": 2 } [/block] [block:api-header] { "type": "basic", "title": "Required parameters" } [/block] [block:callout] { "type": "info", "body": "Dilute uses a single channel pipette to perform the liquid handling. To learn more about single channel liquid handling and the `transfer` command please see [here](https://developers.transcriptic.com/docs/liquid-handling#section-transfer)", "title": "Dilute uses `transfer`" } [/block] [block:parameters] { "data": { "h-0": "Parameter", "h-1": "Explanation", "0-0": "Sample (group)", "2-0": "Volume of sample", "3-0": "Volume of diluent", "4-0": "Tube type for diluted aliquot", "5-0": "Storage condition for diluted aliquot", "1-0": "Container label", "0-1": "A sample group defines all parameters for a single dilution. Additional dilutions may be specified by clicking the 'Add Sample' link at the bottom of the Sample group.", "1-1": "The name for the new resulting diluted aliquot. Aliquot names associated with this container will be derived from the source aliquot name.", "2-1": "The volume of sample to be diluted.", "3-1": "The volume of diluent to be added to the volume of sample to be diluted.", "4-1": "The new container for the newly diluted aliquot. Compatible containers: `micro-1.5`, `micro-2.0`, `96-pcr`, `96-deep`, `96-flat`, `384-pcr`, `384-flat`, `384-echo`.", "5-1": "The storage temperature you require for the diluted sample: either `cold_20`, `cold_4` or `ambient`.", "6-0": "Diluent", "6-1": "Select either a diluent from the reagent library or from your inventory.\n\nFrom the library both water and TE Buffer are available.\n\nFrom your inventory select an aliquot." }, "cols": 2, "rows": 7 } [/block] [block:api-header] { "type": "basic", "title": "Protocol Outputs" } [/block] This protocol outputs a new aliquot of the diluted sample which is stored in your inventory at your specified storage condition. Any aliquots used in the making of the dilutions will be returned to the storage condition from which they came. No measurement data is returned by this protocol. [block:api-header] { "type": "basic", "title": "Recommended guidelines" } [/block] Ensure you are aware of the dead volume in the containers you are using for both the sample to be diluted and the diluent (if you are using a diluent from your inventory). Dead volumes can be found in the containers section [here](https://developers.transcriptic.com/docs/containers#compatible-container-types). Failure to take into account dead volumes can result in insufficient volume being pipetted. [block:api-header] { "type": "basic", "title": "Further reading" } [/block] [block:callout] { "type": "success", "body": "For further advice on your protocol please ask questions to the community at https://forum.transcriptic.com", "title": "Check out the forum if you have more questions." } [/block]